scissile bond

classic Classic list List threaded Threaded
2 messages Options
Reply | Threaded
Open this post in threaded view
|

scissile bond

Dr. Yogendra Sharma
Hi all
Would any body help in letting us to know: how to identify scissile bonds in a protein sequence. Any web based tool or any triplet sequence related with Arg?

Thanks

Yogendra Sharma
_______________________________________________
Proteins mailing list
[hidden email]
http://www.bio.net/biomail/listinfo/proteins
Reply | Threaded
Open this post in threaded view
|

Re: scissile bond

Mark Nance
Yogendra,

You can try Expasy's PeptideCutter:
http://www.expasy.ch/tools/peptidecutter/

The fold of the protein is important, because not all cleavage sites are accessible to proteases.  Conformation is important, too.  There are several protease protection assays where binding of protein / activation / etc. can be monitored by increases or decreases in susceptibility to specific proteases.

Mark


>>> "Dr. Yogendra Sharma" <[hidden email]> 12/04/08 5:58 AM >>>
Hi all
Would any body help in letting us to know: how to identify scissile bonds in a protein sequence. Any web based tool or any triplet sequence related with Arg?

Thanks

Yogendra Sharma
_______________________________________________
Proteins mailing list
[hidden email]
http://www.bio.net/biomail/listinfo/proteins

!DSPAM:63,4938156328201137399876!



_______________________________________________
Proteins mailing list
[hidden email]
http://www.bio.net/biomail/listinfo/proteins