proteins that don't ionize

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proteins that don't ionize

jps-3
Recently purified an unknown protein with a partial N-terminal
sequence match to a bacterial outermembrane protein.  Oddly this
protein does not react with DTNB, does not seem to ionize when mixed
with sinnapinic acid and placed in a MALDI-ToF, and has a molecular
weight of 33kDa as judged by SDS PAGE analysis.

Curious to know if anybody thinks that membrane proteins would have
more difficulty interacting with MALDI matrices than other proteins?
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Re: proteins that don't ionize

PM-21
jps wrote:
> Recently purified an unknown protein with a partial N-terminal
> sequence match to a bacterial outermembrane protein.  Oddly this
> protein does not react with DTNB, does not seem to ionize when mixed
> with sinnapinic acid and placed in a MALDI-ToF, and has a molecular
> weight of 33kDa as judged by SDS PAGE analysis.
>
> Curious to know if anybody thinks that membrane proteins would have
> more difficulty interacting with MALDI matrices than other proteins?

Maybe trie a batch of matrices?
Not all substrates wil "fly" with sinnapinic acid. DHBA? maybe or use
electrospray (TOF) MS.
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