lowry estimation

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lowry estimation

kyoli
hi, all
i m doing lowry estimation of protein and o.d. is coming sometimes
above 1. may i dilute it bt i can't understand when i dilute it o.d.
comes to be very low and in determinig protein content there is no
volume term so how can i specify the amount
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Re: lowry estimation

Dr Engelbert Buxbaum
Am 26.01.2009, 13:06 Uhr, schrieb kyoli <[hidden email]>:

> hi, all
> i m doing lowry estimation of protein and o.d. is coming sometimes
> above 1. may i dilute it bt i can't understand when i dilute it o.d.
> comes to be very low and in determinig protein content there is no
> volume term so how can i specify the amount

Dilute the protein sample before doing the assay.
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