help for designing of primer pair

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help for designing of primer pair

shrinivas Dengeti
hi all...
 i got sequenced one of my enzyme gene ( xylanse) ..whcih contain all
the regulatory elements also
But for cloning and expression studies...i want to get the protein
coding part only na?
so could you help me out in designing  the primers containing the
sites for R.E's also...whcih are also prsent in the vectors ...
 for ex:- pEt series vector... Nco I and xho I are required for
inserting into the vector..

bye take care
With Regarsds
 D.shrinivas
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