anyone here in biology has experience with affinity chromatography?
having problem with my columns recently. my columns worked all right
before, but suddenly didn't enrich proteins. I confirmed the starting
and eluting conditions, and it should be always the same. I heard
before that these antibody columns have a definite life, such as a
number of cycles it can run, but it's unlikely two of my columns stop
working at the same time---one column was very new, and only ran a few
anybody can give a clue? I'm not a prof bio and this
topic is hard to google out. hope some expert friends can share me your
valuable opinions and suggestions (why this happens... solutions...).