antibody affinity chromatography question

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antibody affinity chromatography question

zhjwmy
Hi there,
anyone here in biology has experience with affinity chromatography?
I'm
having problem with my columns recently. my columns worked all right
before, but suddenly didn't enrich proteins. I confirmed the starting
and eluting conditions, and it should be always the same. I heard
before that these antibody columns have a definite life, such as a
number of cycles it can run, but it's unlikely two of my columns stop
working at the same time---one column was very new, and only ran a few
times.

anybody can give a clue? I'm not a prof bio and this
topic is hard to google out. hope some expert friends can share me your
valuable opinions and suggestions (why this happens... solutions...).
much appreciated!



     
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