Re: Western Blot Formalin Fixed Tissue

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Re: Western Blot Formalin Fixed Tissue

Dr Engelbert Buxbaum
In article <[hidden email]>,
[hidden email] says...
>
> I would like to know if it is possible to do western blotting experiment
> using formalin fixed frozen tissues of rat brain, thanks for that Rong

Difficult, as formalin cross-links proteins. The more common method is
to rehydrate sections and elute proteins with hot RIPA-buffer containing
detergents. The proteins are reduced, carboxymethylated and protease
digested. The resulting peptides are separated by capillary
electrophoresis and identified by MS/MS. For a review see Nirmalan et
al.: Mol. Biosyst. 4 (2008) 712-20
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