Re: Phe Auxotrophic E. Coli BL21(DE3)

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Re: Phe Auxotrophic E. Coli BL21(DE3)

Clement Angkawidjaja
Hi,

The pheA-deleted mutant of E coli K12 (strain JW2580) is available for
purchase from the National Bioresource project, Japan
(http://www.shigen.nig.ac.jp/ecoli/strain/top/top.jsp). You can order it
online.

You can introduce lambda-DE3 using the lambda-DE3 lysogenation kit provided
by Novagen.

Hope this helps.

Clement Angkawidjaja, Ph.D
Specially Appointed Assistant Professor
Laboratory of Molecular Biotechnology
Division of Advance Science and Biotechnology
Osaka University
GSE Common East 8F
2-1 Yamadaoka, Suita-shi, Osaka, 565-0871, Japan
Tel/fax: +81-6-6879-4580

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Subject: Proteins Digest, Vol 47, Issue 4


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> Date: Mon, 13 Apr 2009 15:01:45 -0400
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> Hello, I'm looking for a Phe auxotrophic strain of E Coli that can be
> used with plasmids with T7 promoter. Preferably BL21 (DE3). Can
> anybody direct me to a bank, lab or were to buy it? Thnxs
>
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