Protein transfer from agarose gel

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Protein transfer from agarose gel

Thomas R. Brown
Bryan

Have you had any luck with "western blots" from agarose to membranes?

Tom Brown





Thomas R. Brown, Ph.D
Senior Scientist
Oxford Biomedical Research, Inc.
2165 Avon Industrial Drive
Rochester Hills, MI 48309
Ph: 248.852.8815  ext 19
Fax. 248.852.4466

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Re: Protein transfer from agarose gel

Dr Engelbert Buxbaum
Am 04.02.2010, 14:40 Uhr, schrieb Thomas R. Brown  
<[hidden email]>:

> Have you had any luck with "western blots" from agarose to membranes?

That should not pose any difficulty at all, since agarose pores are larger  
than those of acrylamide. Hence any method working with the latter should  
also work with the former.

Meth. Mol. Biol. 536 (2009) deals with blotting techniques.
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