MRC-5(ATCC CCL171) cell culture

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MRC-5(ATCC CCL171) cell culture

Dear Sir:


I have found the letter as follol from the internet.

Just I am learning how to improve the MRC-5 cell density in T175 Flask.

Could you help me ?


Thank you very much




Dear Sir!

I have another question to you.

When I culture MRC-5(CCL-171) in 60mm dish and 100mm dish, we can get

satisfied cell number,

2,000,000 cells/60mm dish with 3ml DMEM(low glucose,10% FBS) and 6

~7,000,000 cells/100 dish with10ml DMEM(low glucose,10% FBS)

But I can not get enough cell number in 150mm dish and 490cm2 roller


8,000,000 cells/150mm dish with 15~20ml DMEM(low glucose,10% FBS)

and 20,000,000 cells/490cm2 roller vessel with 50~100 ml DMEM(low

glucose,10% FBS).I have done these test at passage number 26 and 27.

I want  get much more cell number same area in 490cm2, at least


when I plating 1~1,500,000cells. How to overcome this problem.

Could you please inform to me about that or introduce a expert of MRC-5


Thank you .


With best regard.




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2nd CFP ICARIS 2012 - new track in Computational Immunology, Immunoinformatics & Systems Immunology

Mario Pavone


New Track in Computational Immunology, Immunoinformatics & Systems Immunology
28-29 August 2012 - Taormina, Italy


                         **Apologies for cross-posting**
          The 11th International Conference on Artificial Immune Systems
                       August 28-31, 2012 Taormina, Italy
                             [hidden email]


Important Dates:
Paper submission:  1st  March 2012
Author notification:  15th May 2012
Paper final version: 15th June 2012

General Information:
ICARIS 2012 aims to build on the success of previous years to provide  
a forum for AIS researchers in academia and industry to present and  
discuss their latest advances. In addition to peer-reviewed papers,  
ICARIS 2012 will present a range of plenary lectures and tutorials to  
inspire and facilitate both the computer scientists and immunologists  
in their work.

ICARIS 2012 is divided into two main tracks:
* 28 and 29 August 2012: Computational Immunology & Immunoinformatics
(for Oral Presentations & Posters submission)

* 30 and 31 August 2012: Immunological Computation, Immune-inspired  
Immune-inspired Heuristics                 
         (for Full papers submission)
(for Oral Presentations & Posters submission)

- Track on Computational Immunology

This track aims is to attract researchers from computer science,  
information science, engineering, biomathematics, biomedicine,  
bioinformatics, systems biology, and immunology to share and exchange  
their knowledge of state-of-the-art research issues, methodologies,  
ideas, and challenges.

Topics of interest include:
* Immune modelling;
* Structural Immunoinformatics;
* Epitope analysis and prediction;
* Analysis and prediction of MHC-peptide binding;
* Databases and ontologies for Immunoinformatics;
* Analysis and prediction of minor histocompatibility antigens;
* Predictive models on organ transplantation;
* Immunogenomics;
* Vaccine design;
* Multi-agent based models of immunity;
* Gene networks and systems biology in immunity;
* Allergenicity prediction.

In this track, authors are requested to submit an abstract following  
the instructions on ICARIS 2012 website:

The abstract submissions must be done no later than the submission  
deadline:  1 March 2012. All accepted abstracts will be published in a  
book of abstracts. Full versions of the best works on this track will  
be published in the Immunomics Research Journal and BMC Bioinformatics.

- Track on Immunological Computation, Immune-inspired Engineering,  
Immune-inspired Heuristics

This track is for papers describing applications to computational and  
engineering problems, and theoretical aspects (e.g. mathematical  
modelling of algorithms, convergence analysis, empirical investigation  
into algorithm performance and complexity analysis) of AIS. For the  
first class, the problem domain should be clearly explained, detailing  
why it is challenging and why AIS are a suitable methodology to use.  
Experiments should be carefully explained, with the expectation that  
appropriate statistical analysis of results will be used to help draw  
conclusions. Where possible, the approach taken should be compared  
with alternative strategies. In order to appeal to the widest  
audience, theoretical papers must ensure that mathematical arguments  
and backgrounds are clearly presented, and the paper should also  
discuss the practical implications of any theoretical results.

In this track authors may submit their manuscripts in two different  
ways: abstract paper, or regular paper (as usual tradition).

* Abstract paper
Authors must follow the instructions described on ICARIS 2012 website:

* Regular paper
All papers will undergo a double blind review process, and they should  
be no longer than fourteen (14) pages. Is recommended that authors  
name and affiliation should not appear on the paper. Authors are  
requested to submit a PDF file at the link following the  
formatting instructions at the Springer-Verlag LNCS site, no later  
than the submission deadline: 1 March 2012.

All accepted papers, as for the previous editions, will be published  
in Springer's series Lecture Notes in Computer Science. The authors of  
best (regular) papers on this track will be invited to extend their  
work for publication in an international journal (TBA).

During the submission authors must make clear which stream the paper  
should to be considered for. You will be provided a reference number  
that will be used throughout the submission process.

We look forward to welcoming you to Taormina in August 2012.
C. Coello Coello, J. Greensmith, N. Krasnogor, P. Lio', G. Nicosia, M. Pavone.

Dr. Mario Pavone (PhD)
Assistant Professor
Department of Mathematics and Computer Science
University of Catania
V.le A. Doria 6 - 95125 Catania, Italy
tel: 0039 095 7383038
fax: 0039 095 330094
Email: [hidden email]

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