I am trying to separate 3 smalls peptides (15kDa, 9kDa and 7kDa) , I am using the technic of "tricine sds-page" by schagger H. et al. of Nature protocols
The difference between the 9kDa and 7 kDa is visible but I want to increase it, the problem is that the system of detection ( fluorescence) cannot work with gel of 16% of acrylamide so i am working on gel 10%, and i am going to see if i can go further in the percentage of acrylamide.But my problem is about the voltage that I apply to the gel:
On the net i can't find any informations about " how to determine the best voltage/current in a sds-page gel"
So how can I determine the best voltage/current to apply to my gel?