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Im also sequencing PCR-Products using Alf Express.
I clean the PCR-Product from the old primers using the PCR-kit from GE.
This product is used directly without any ss-ends.
Instead of cy5-dATP I have sequencing primers 5labeled with cy5.
The sequencing primer are positioned like a nested PCR-primer a little bit
away from the end of the PCR product.
The sequencing reaction is carried out using the Sequencing-Kit from GE.
If you are interested in the protokoll, please let me know.
Saluti and ciao
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